Senior Associate, Engineer
19 Presidential Way, Suite 202 Woburn, MA 01801 US
As part of the Upstream Process Development Team, the Senior Research Associate/Research Associate II is expected to lead, design, and conduct experiments aimed at defining robust bioreactor processes for the production of AAV vectors in HEK293 and/or HeLa Producer cell platforms and help develop robust and scalable processes suitable for cGMP production. This role will encompass unit operations which are typically seen in the upstream function and require cross-collaboration with downstream as well. A successful candidate will have technical knowledge and experience in upstream process development and a drive to learn.
• Semi-independently lead project/program experiments including planning and executing experiments across the Upstream Process Development team for AAV production working closely with senior scientists. Troubleshooting the process and equipment when needed with associate scientists in the lab is expected.
• Design and apply DOE studies to develop, refine, optimize and characterize cell culture and vector production processes.
• Apply both scientific and engineering concepts to develop and scale the mammalian cell culture processes, including transient transfection and infection-based processes.
• Contribute to development of platforms/programs to enhance AAV manufacturing technologies, capabilities and processes (such as media development, bioreactor fed-batch and perfusion process development, optimization and scale-up).
• Generate, manage, evaluate, and maintain critical data in a highly organized manner. Help improve and maintain ELN templates and complete experiment write-ups in timely manner. Author technical reports, tech transfer documents, and prepare scientific presentations as needed. Write/revise SOPs as needed.
• Must demonstrate a keen knowledge of experimental design, underlying scientific principles, and the ability to analyze and assess results.
• Strong team player-Must work well within teams in highly collaborative environment, where equipment and space are shared with wider pharmaceutical development team
For SRA: BS in Biochemical engineering or Biological Sciences with 4+ years, or MS with 2+ year of industrial experience in mammalian cell culture process development.
For RAII: BS in Biochemical engineering or Biological Sciences with 2+ years, or MS with 0+ year of industrial experience in mammalian cell culture process development.
• Experience with small- or large-scale single-use bioreactor operations, and technology transfer to pilot plant or cGMP manufacturing for clinical stage products is preferred.
• Experience with development of viral vectors, live viral vaccines, producer cell lines, high throughput or disposable bioreactors, culture media development, or perfusion unit operations is preferred. Familiarity with depth-filtration, normal flow filtration, tangential flow filtration, and membrane filtration would be an additional strength.
• Should be highly motivated, have excellent organizational and communication skills, and be able to multitask in a fast-paced environment with changing priorities.
• Ability to execute and follow-through to completion and documentation. Ability to work effectively in a collaborative team environment where results are achieved through influence and the incorporation of multiple points of view.
Pay 40-48/hr based on experience